Abstract
Valencia, J., Yeste, M., Quintero-Moreno, A., del Pilar Niño-Cárdenas, C., & Henao, FJ (2020).
Variation between and within boar ejaculates in their ability to withstand freezing-thawing is a limitation for sperm cryopreservation. Consequently, the search for freezability markers is imperative not only in sperm but also in seminal plasma (SP). The present study aimed to evaluate the relationship between cholesterol content, the relative levels of NPC2 and AQN-1 at two different retention times (0 h: HT0 and 24 h: HT24) at 17 °C, and the freezability of the boar sperm 45 ejaculates were cryopreserved and subsequently classified as having good (GFE) or poor (PFE) freezability based on their sperm viability after thawing and their total motility. Before cryopreservation, the relative abundance of two SP proteins (NPC2 and AQN-1) and cholesterol content in sperm and SP were determined by immunoblotting and colorimetric methods, respectively. These determinations were carried out after ejaculation (HT0) and after 24 h of storage at 17 °C (HT24).